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How does a Laboratory Autoclave Work?

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Before getting into the how does a laboratory autoclave work, we will be seeing a brief about what is autoclave and it’s basic. What is Autoclave? The autoclave is to most common equipment you can find in the laboratory in vitro cultures. In other words, Autoclave is equipment used to sterilize various solutions, objects, containers, etc. It increases the pressure and hence the temperature is raised above the normal point for sterilizing process. How does autoclave work for Laboratory? The working of an autoclave is common as a normal pressure cooker we use in the kitchen. There are variously sized autoclaves available. Working of all of them is common. it closes the lid or chamber of the equipment. Steam is replaced in place of air to start the sterilization process. Basically, the cycle for the working of Autoclave consists of 3 different phase. The three-phase does the same process as we mentioned above. The three phases are – Purge Phase – Exposure (Sterilization...

Sterilization of ETO and Hydrogen Peroxide on Low Temperature

There are a lot of methods and techniques available for  steam sterilization  and steam quality on High temperature. Now, the point remains the same on the low temperature. In this post, we will discuss the sensitive and moisture sensitive load on low Temperature. We all know to destroy bacteria, fungi and spores we can use the Steam method which is very effective. Not only the steam method is cost-effective but also is simple to do. The problem here is that the same method cannot be used on all types of materials and instruments. Some of those materials include Heat and moisture sensitive materials. One can not apply the steam method for that. For this reason, we need an alternate method to do the same. EtO Gas and Life Cycle EtO gas is carcinogenic, explosive and mutagenic. The gas is colorless and is Poisonous. It is used to destroy the nucleic acid of all the microorganisms and cellular proteins. The gas attacks the microorganisms but along with this, it ca...

Physical and Chemical Method of Sterilization

Sterilization is an amazing technique that ensures no medical and surgical instruments transmit bacterial infections to patients. Whether it is a virus, bacteria, microorganism or bacterial spores,  sterilization techniques  are applied to destroy all sorts of bacteria permanently.  The basic techniques of sterilization are listed below. 1. Physical Method (a) Thermal (Heat) methods (b) Radiation method (c) Filtration method 2. Chemical Method Physical Method WET HEAT  Wet heat method is the autoclaving process, which is the most efficient method of sterilization techniques. It is employed in micro labs for the prevention of disease-causing bacteria. Whether it is spores, virus, bacteria or microorganism, Autoclaving method is an ideal option, which is why it is the user-friendly sterilization techniques. In this process, pressurized steam is applied to the substance that is to be sterilized. This is an effective process of  sterilization for...